Because nitrous oxide and oxygen sedation has become a mainstay of medical and dental practice, researchers have been able to study it across many decades. They know how nitrous oxide and oxygen sedation affects very young patients, pregnant women and the elderly. They have documented reactions that occur between nitrous and the medications millions of people take every day. This careful research has revealed that nitrous oxide and oxygen sedation is extremely safe for the vast majority of people.
Among the most famous studies is that conducted by Dr. Gardner Colton of New York's Columbia University. Colton documented more than 193,000 cases without a fatality. The work of Dr. Niels Bjorn was even more striking. He followed over 4 million cases, again without finding a single death.
Nitrous oxide and oxygen sedation carries few if any side effects and produces no known allergic response. For these reasons it makes a nearly ideal form of anxiety relief in the contemporary dental office.